Resources

Plasmids

Channel Vector Type Promoter Reporter Parent Lab Resource
TRP-4 Worm Plasmid sra-6 Chalasani [Addgene]
TRP-4 Worm Plasmid odr-3 Chalasani [Addgene]
hsTRPA1 Mammalian pDNA CMV myc-tag Chalasani [Addgene]
hsTRPA1 Mammalian AAV hSyn tdTomato Chalasani [Addgene]

*Note: For antibiotic resistance and detailed maps, please click the “Addgene” link for each construct

Hardware

Delivering Ultrasound:

The Core Setup

Component Recommendation Function
Signal Generator Keysight 33600A Series Generates the raw waveform
RF Power Amplifier Electronics & Innovation 2100L Amplifier Boosts low-voltage signal from the generator to the levels required to drive the piezo element
Transducer Custom American Piezo Ceramics transducer* The “Speakers.” Converts electrical energy into mechanical sound waves

*Note: It is practically impossible to list a “universal” transducer. Frequency (e.g., 0.5-10 MHz), focal length, and element size are highly dependent on your specific experimental needs.

Measuring Ultrasound: Performance & Verification

Acoustic Characterization

  1. Fiber-optic Hydrophone System (Precision Acoustics): the gold standard for measuring peak-to-peak pressure and mapping focal zones.

Electrical Monitoring

  1. Oscilloscope (Keysight DSOX2024A): Visualizes real-time voltage and current waveforms.
  2. Voltage Probe (Keysight N2863B, Passive Probe): Measures amplitude delivered directly to the piezo.
  3. Current Probe (Tektronix CT2 AC): Enables non-invasive current measurement to calculate real-time acoustic impedance.

Miscellaneous Hardware & Connectivity

  1. 50-Ohm BNC cables and BNC T-adapters (Federal Custom Cable, [Full List of Cables])
  2. FX888D-23BY Digital Soldering Station (Hakko)
  3. Aquasonic Gel & Degassed Water
  4. 3D printed files: Contact us for STL files and other blueprints